RT-PCR HLA Typing
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What is QTYPE HLA Typing?
For samples that need to be processed fast, QTYPE offers rapid and accurate real-time PCR-based HLA typing for 11 loci with a low-to-intermediate and single-antigen bead resolution.
- Rapid: RT-PCR HLA typing in less than 1 hour with <10 mins of hands-on time
- High robustness: Each allele covered by multiple unique reactions
- Streamlined: Single-step workflow and automated data analysis
- Single test for 11 loci: HLA-A, B, C, DRB1, DRB3,4,5, DQA1, DQB1, DPA1, DPB1
- Enhanced resolution: Single antigen bead (SAB) resolution and expandable primer/probe design
- Covers all alleles from the database used in design
- Direct to high resolution for DPB1 CWDs
A Complete Workflow that Offers Speed and Precision in Real Time
Simple and Rapid Workflow: HLA Typing Within an Hour
QTYPE plates can be set up in batches and stored in the dark at +4°C to +8°C for up to 8 hours before performing the real-time PCR run.
QTYPE Kit Overview
- 384-well plate with pre-dispensed, dried reaction mixes
- Each well contains at least one HLA reaction and an internal amplification control reaction
- Each reaction consists of at least two SSP primers and a TaqMan probe
- Ready to use, single run aliquot of master mix complete with Taq polymerase, dNTPs and buffer
- Optically clear sealing sheets
Instrumentation Compatibility
Real-time PCR instruments that have been validated for use with QTYPE assays.
How does QTYPE HLA Typing Work?
QTYPE is a rapid RT-PCR HLA typing solution, designed to provide results within one hour. Kits include a DNA well plate pre-aliquoted with dried primers and probes that are designed to bind with select alleles.
- QTYPE utilizes primer and probe combinations to enhance resolution
- Specificity provided by primers and sequence-specific TaqMan probes (i.e. dual control of specificity)
- No need for additional verification steps (e.g. melt curve analysis)
Flexible multiplexing for increased resolution
- Future-proof design: significant capacity to add new reactions and increase resolution due to probe-based technology
- Two channels are used for HLA-specific reactions-class I/II, one channel for an internal amplification control and one channel for reference dye
- Reaction is monitored in real time, which allows for increased resolution
SCORE™ 6 Provides Fast Results with Automated Analysis
Seamless Analysis
Simple workflow to import and analyze QTYPE data. Results are calculated automatically, which allows for objective analysis.
Transparency of Results
Virtual plate view, allowing to look up any of the real time data generated by the reactions. Easy to view all result combinations.
Convenience
Easy to learn and access. Streamlined workflow for on-call typing.
Safety
Several user groups with different permission level. Can maintain two SQL server databases, which can be backed up and restored.
Test Kit Ordering Details
| Product number | Product name | Tests Per Kit |
|---|---|---|
| 201.701-03 | OLERUP QTYPE 11 KIT | 3 |
| 201.701-10 | OLERUP QTYPE 11 KIT | 10 |
| Product number | Product name | Tests Per Kit |
|---|---|---|
| 201.701-03 | OLERUP QTYPE 11 KIT | Warning: Undefined array key “column_3_class” in /home/stagecaredx/public_html/wp-content/themes/caredx/blocks/block-table-with-three-columns.php on line 114 |
| Product number | Product name | Tests Per Kit |
|---|---|---|
| 203.501-01 | Color Compensation Calibration Plate | 1 |
| 203.502-10 | Optically-clear PCR sealing sheets | 10 |
| Product number | Product name | Tests Per Kit |
|---|---|---|
| 203.501-01 | Color Compensation Calibration Plate | Warning: Undefined array key “column_3_class” in /home/stagecaredx/public_html/wp-content/themes/caredx/blocks/block-table-with-three-columns.php on line 114 |
Olerup QTYPE 11 and SCORE 6 are available as CE-IVD and research use only products. Research Use Only products are not to be used for diagnostic procedures. For local regulatory status, please contact CareDx. Olerup QTYPE 11 is referred to as QTYPE in this document. © 2025 CareDx, Inc. All service marks or trademarks are owned or licensed by CareDx, Inc. or its affiliates. All rights reserved.
